As long as you didn't bust them out of their trees and then go chasing after them and bust them again, you shouldn't have bothered the roost area any. As far as their memory goes, I'm on the fence about that. In the fall you want to bust them up and follow the least number of birds a short distance, set up and call them back together. It's easier to call a lone bird then it is to call 3-4, safety in numbers. Yet they seem to remember the sound of a call for a long time, if you call a bird in and you get busted you'll have a hard time getting that bird to respond to that call for a long time? That's why if you do any pre season calling don't use a call that you plan to use for hunting, they remember sound very very well.
Dodge County NWTF Chapter
WI. Youth Turkey Mentor