I do not seperate the reeds on my mouth calls....I wash them off..give em a shake..and store in the fridge....Upon use just a min or so and the top reed seprates for use..i like the middle and bottom reed to remain stuck as long as possible....
I usually separate the top reed with either a plastic hair comb tooth or a plastic coated paper clip. I also let the other reeds stick. For me I think it gives a better sound,but that's all personal preference.As for the box and slate I use 220 sand paper as needed to clean be sure not sand to much on the box as it will change the sound, re-chalk and you're ready to go.Glass or aluminum get 100 grit for me. You can clean striker tips with 220 also.
I to feel there is an increase in sound by letting the two reeds stick..I have also come to the conclusion that by not seperating the top reed..my calls last longer because there is no stress placed on the top reed..imo
another thing I should mention I always put mouth calls in fridge for storage.Here is another little tip, if you would like a little more rasp from a mouth call instead of placing the call flat in the roof of your mouth cant it just a little bit.It seems to work with any mouth call.[;)]
I sometimes put mouth calls on 50/50 mouthwash water solution for a hour or so to disinfect, but I never leave them in there any longer than that.Rinse,dry,fridge.To me and this is just my opinion a wet,saturated mouth call has a dead sound to it.If thats been working for you have at it.